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Literature summary extracted from
- Simple and efficient synthesis of 5' pre-adenylated DNA using thermostable RNA ligase (2011), Nucleic Acids Res., 39, e117.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 6.5.1.3 | synthesis | efficient synthesis of 5' pre-adenylated DNA | Methanothermobacter thermautotrophicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.5.1.3 | Methanothermobacter thermautotrophicus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.5.1.3 | additional information | the enzyme catalyzes an adenylation reaction that is specific for ATP and either ssDNA or RNA. It does not require synthesis of a template strand for adenylation. To achieve quantitative DNA adenylation, single turnover reaction condition with substrate to enzyme ratio 1:1 should be used | Methanothermobacter thermautotrophicus | ? | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.3 | 60 | 65 | - |
Methanothermobacter thermautotrophicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.3 | 60 | 65 | optimal for stability | Methanothermobacter thermautotrophicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.3 | 6 | 6.5 | in the presence of Mg2+, the reaction has a pH optimum of 6.06.5 | Methanothermobacter thermautotrophicus |
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Release 2023.1 (January 2023)
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